| 郑秋灵,陈新国,包瑶萍,李燕贤,熊亮.高效液相色谱-电雾式检测器测定氨溴特罗口服液中羟乙基纤维素含量[J].中国药品标准,2023,24(1): |
| 高效液相色谱-电雾式检测器测定氨溴特罗口服液中羟乙基纤维素含量 |
| Determination of Hydroxyethyl Cellulose in Ambroxol Hydrochloride and clenbuterol hydrochloride oral solution by high-performance Liquid chromatography-charged aerosol detector (HPLC-CAD) |
| 投稿时间：2022-09-01 修订日期：2023-01-31 |
| DOI： |
| 中文关键词: 电雾式雾式检测器 药用辅料 羟乙基纤维素 高效液相色谱法 |
| 英文关键词: Charged aerosol detector (CAD) Pharmaceutical excipients Hydroxyethyl Cellulose HPLC |
| 基金项目: |
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| 摘要点击次数: 453 |
| 全文下载次数: 139 |
| 中文摘要: |
| 目的：建立测定氨溴特罗口服液中羟乙基纤维素的高效液相色谱-电雾式雾式检测器(HPLC-CAD)方法。方法：采用TSKgtl G2000SW (60cm×7.5mm，10 μm)，以10 mmol/L乙酸铵(pH4.5):乙腈=95:5为流动相，柱温：30 ℃，流速：1.0 mL/min，电雾式检测器的蒸发温度为50 ℃。结果：该方法对羟乙基纤维素专属性良好，精密度、平行性和回收率均满足分析要求，峰面积线性关系良好(r＞0.995)。结论：新建方法专属性好、操作简便，可以满足氨溴特罗口服液中羟乙基纤维素分析的要求。 |
| 英文摘要: |
| Objective：To establish a high-performance Liquid chromatography-charged aerosol detector (HPLC-CAD) method for the determination of Hydroxyethyl Cellulose in Ambroxol Hydrochloride and clenbuterol hydrochloride oral solution. Method： the chromatographic column was TSKgtl G2000SW column (60cm×7.5 mm, 10 μm). The mobile phase was 10 mmol/L ammonium acetate (pH4.5)/acetonitrile (95:5, V/V). The flow rate was 1.0 mL/min, and the column temperature was 30℃. The evaporator temperature of CAD was 50℃. Result：This method can separate Hydroxyethyl Cellulose well and has good specificity. The precision, repeatability, and recovery of this method can meet the analysis requirements. There was a good Linearity among the Hydroxyethyl Cellulose (r＞0.995). Conclusion：The established method has good specificity and of high maneuverability, which can be used for quantitative analysis of Hydroxyethyl Cellulose analysis in Ambroxol Hydrochloride and clenbuterol hydrochloride oral solution. |
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