| 刘懋,笔雪艳.一测多评法同时测定陈皮中6个黄酮类成分的含量[J].中国药品标准,2023,24(2): |
| 一测多评法同时测定陈皮中6个黄酮类成分的含量 |
| Simultaneous determination of six Flavonoids in Citri Reticulatae Pericarpium by quantitative analysis of multi-components by single marker |
| 投稿时间：2022-07-05 修订日期：2023-03-29 |
| DOI： |
| 中文关键词: 陈皮 一测多评 相对校正因子 橙皮苷 含量测定 香蜂草苷 川陈皮素 橘皮素 |
| 英文关键词: Citri Reticulatae Pericarpium quantitative analysis of multi-components by single-marker(QAMS) relative correction factors（RCFs） hesperidin content determination didymin nobiletin tangeretin |
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| 中文摘要: |
| 摘要 目的：建立一测多评法同时测定陈皮中6个黄酮类成分的含量。方法：采用OSAKA CAPCELL PAK C18色谱柱（250 mm×4.6mm，5μm），流动相为乙腈-0.1%甲酸溶液，梯度洗脱；流速为1.0 mL?min-1，柱温为30°C，检测波长为330 nm。以橙皮苷为内参物，计算橙皮苷与芸香柚皮苷、香蜂草苷、川陈皮素、3,5,6,7,8,3′,4′-七甲氧基黄酮、橘皮素之间的相对校正因子，分别采用一测多评法和外标法对15批陈皮中6个黄酮类成分的含量进行测定并比较结果。结果：6种成分在各自范围内线性关系良好（r≥0.9999）；平均加样回收率99.95%~102.97%，RSD 0.81%~2.03%。一测多评法与外标法的含量测定结果无显著性差异（P>0.05）。结论：该方法操作便捷、准确可靠、重复性好，可用于陈皮的质量控制。 |
| 英文摘要: |
| Abstract objective: To establish a method for determination of six Flavonoids in Citri Reticulatae Pericarpium by quantitative analysis of multi-components by single marker. Methods:A OSAKA CAPCELL PAK C18 column was used（250 mm×4.6mm，5μm）, with acetonitrile -0.1% formic acid as mobile phase with gradient elution, The flow rate was set at 1.0 mL·min-1，the temperature maintained at 30 ℃，and the detection wavelength was set at 330 nm. Hesperidin was chosen as the internal reference substance to caculate the relative correction factors（RCFs）of narirutin,didymin,nobiletin,3,5,6,7,8,3"",4""-heptamethoxyflavone,tangeretin. Using relative retention time method and external standard method（ESMS）to determinate the contents of 6 Flavonoids in 15 batches of Citri Reticulatae Pericarpium and compare the results. Results：The linear relationships of six component were well (r＞0.9999). The average recoveries were 99.95%~102.97% with RSDs of 0.81%~2.03%. There were no significant differences（P>0.05） between the calcurlated value and estimated value on QAMS and external standard method of content determination. Conclusions：The method is accurate and reliable，sensitive，reproducible and easy to operate. and can be applied to the determination of Citri Reticulatae Pericarpium and control the quality. |
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