文章摘要
周海艳,高云佳,王敏,张庆英,屠鹏飞.UPLC同时测定不同产地白术中3种白术内酯成分的含量[J].中国药品标准,2023,24(1):
UPLC同时测定不同产地白术中3种白术内酯成分的含量
Simultaneous Determination of Three Atractylenolides in Atractylodes macrocephala by UPLC
投稿时间:2021-10-21  修订日期:2023-01-31
DOI:
中文关键词: 白术  白术内酯Ⅲ  白术内酯Ⅱ  白术内酯Ⅰ  超高效液相色谱  聚类分析  主要成分分析
英文关键词: Atractylodes macrocephala  Atractylenolide III  Atractylenolide II  Atractylenolide I  UPLC  cluster analysis  principal component analysis
基金项目:国家重点研发计划(2018YFC1707904,2018YFC1707900)
作者单位E-mail
周海艳 北京大学 药学院 天然药物及仿生药物国家重点实验室北京华医神农医药科技有限公司 liliaceae@126.com 
高云佳 北京大学 药学院 天然药物及仿生药物国家重点实验室北京华医神农医药科技有限公司  
王敏 北京华医神农医药科技有限公司  
张庆英 北京大学 药学院 天然药物及仿生药物国家重点实验室 qyzhang@ bjmu.edu.cn 
屠鹏飞* 北京大学 药学院 天然药物及仿生药物国家重点实验室 pengfeitu@bjmu.edu.cn 
摘要点击次数: 398
全文下载次数: 37
中文摘要:
      目的:建立UPLC同时测定白术中3种白术内酯成分含量分析方法。方法:样品以甲醇超声提取30 min进行处理。采用超高效液相色谱,以Agilent Poroshell 120 EC-C18(3.0 mm×150 mm,2.7 μm)为色谱柱,流动相水(A)-甲醇(B)梯度洗脱,流速0.5 mL?min-1,柱温30℃,检测波长220 nm和276 nm,进样量3 μL。采用IBM SPSS 27.0软件对24批白术进行聚类分析和主成分分析。结果:3个成分在考察的浓度范围内,浓度与峰面积之间呈良好的线性关系(r﹥0.999),回收率均在103.05%~106.35%,RSD 2.0%~3.3%。经聚类分析发现,24批白术药材可聚为四大类;经主成分分析发现,2个主成分因子的累计方差贡献率为98.975%(第一主成分、第二主成分的贡献率分别为92.124%、6.601%)。结论:该方法简便、快捷、准确且灵敏度高,对评控白术药材的质量提供参考。
英文摘要:
      Objective: To establish a UPLC method for the simultaneous determination of three Atractylenolides in Atractylodes macrocephala. Method: The samples were extracted with methanol for 30 minutes by ultrasonic method .Three compounds in A. macrocephala were simultaneously determined by UPLC with Agilent Poroshell 120 EC-C18(3.0 mm×150 mm,2.7 μm),with water ( A) - methanol ( B) as the mobile phase for gradient elution; at the flow rate of 0.5 mL?min-1; the determination wavelength was 220 nm and 276 nm; and the injection volume was 3 μL. Cluster analysis and principal compoent analysis were performed by IBM SPSS 27.0 statistical software. Result: There was a good linear relationship between the concentration and peak area of all the three components in the investigated concentration range ( r>0.999) . The average recoveries ranged from 103.05% to 106.35% with RSD of 2.0%-3.3%. Through cluster analysis, 24 batches of A. macrocephala could be clutered into 4 groups. According to the principal component analysis,the cumulative variance contribution rate of the two principal component factors was 98.975%(contribution rates of first principal components and second principal components were 92.124% and 6.601%).Conclusion: The method is simple,rapid,accurate and highly sensitive to provide a reference for quality evaluation of A. macrocephala.
  查看/发表评论  下载PDF阅读器
关闭