| 周海艳,高云佳,王敏,张庆英,屠鹏飞.UPLC同时测定不同产地白术中3种白术内酯成分的含量[J].中国药品标准,2023,24(1): |
| UPLC同时测定不同产地白术中3种白术内酯成分的含量 |
| Simultaneous Determination of Three Atractylenolides in Atractylodes macrocephala by UPLC |
| 投稿时间：2021-10-21 修订日期：2023-01-31 |
| DOI： |
| 中文关键词: 白术 白术内酯Ⅲ 白术内酯Ⅱ 白术内酯Ⅰ 超高效液相色谱 聚类分析 主要成分分析 |
| 英文关键词: Atractylodes macrocephala Atractylenolide III Atractylenolide II Atractylenolide I UPLC cluster analysis principal component analysis |
| 基金项目:国家重点研发计划（2018YFC1707904，2018YFC1707900） |
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| 中文摘要: |
| 目的：建立UPLC同时测定白术中3种白术内酯成分含量分析方法。方法：样品以甲醇超声提取30 min进行处理。采用超高效液相色谱，以Agilent Poroshell 120 EC-C18（3.0 mm×150 mm，2.7 μm）为色谱柱，流动相水（A）-甲醇（B）梯度洗脱，流速0.5 mL?min-1，柱温30℃，检测波长220 nm和276 nm，进样量3 μL。采用IBM SPSS 27.0软件对24批白术进行聚类分析和主成分分析。结果：3个成分在考察的浓度范围内，浓度与峰面积之间呈良好的线性关系（r﹥0.999），回收率均在103.05%~106.35%，RSD 2.0%~3.3%。经聚类分析发现，24批白术药材可聚为四大类；经主成分分析发现，2个主成分因子的累计方差贡献率为98.975％（第一主成分、第二主成分的贡献率分别为92.124％、6.601％）。结论：该方法简便、快捷、准确且灵敏度高，对评控白术药材的质量提供参考。 |
| 英文摘要: |
| Objective: To establish a UPLC method for the simultaneous determination of three Atractylenolides in Atractylodes macrocephala. Method: The samples were extracted with methanol for 30 minutes by ultrasonic method .Three compounds in A. macrocephala were simultaneously determined by UPLC with Agilent Poroshell 120 EC-C18（3.0 mm×150 mm，2.7 μm），with water ( A) - methanol ( B) as the mobile phase for gradient elution; at the flow rate of 0.5 mL?min-1; the determination wavelength was 220 nm and 276 nm; and the injection volume was 3 μL. Cluster analysis and principal compoent analysis were performed by IBM SPSS 27.0 statistical software. Result: There was a good linear relationship between the concentration and peak area of all the three components in the investigated concentration range ( r＞0.999) . The average recoveries ranged from 103.05% to 106.35% with RSD of 2.0%-3.3%. Through cluster analysis, 24 batches of A. macrocephala could be clutered into 4 groups. According to the principal component analysis，the cumulative variance contribution rate of the two principal component factors was 98.975%（contribution rates of first principal components and second principal components were 92.124% and 6.601%）.Conclusion: The method is simple，rapid，accurate and highly sensitive to provide a reference for quality evaluation of A. macrocephala. |
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