文章摘要
蒋健.HPLC法同时测定风寒感冒颗粒中苦杏仁苷、葛根素和橙皮苷的含量[J].中国药品标准,2019,20(6):482-485
HPLC法同时测定风寒感冒颗粒中苦杏仁苷、葛根素和橙皮苷的含量
Simultaneous Determination of Amygdalin,Puerarin and Hesperidin in Fenghan Ganmao Granules by HPLC
投稿时间:2019-02-14  修订日期:2019-11-26
DOI:
中文关键词: 风寒感冒颗粒  苦杏仁苷  葛根素  橙皮苷  高效液相色谱法
英文关键词: Fenghan Ganmao Granules  amygdalin,puerarin,hesperidin  HPLC
基金项目:
作者单位E-mail
蒋健 南京市食品药品监督检验院 jiangjane1982@sina.com 
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中文摘要:
      目的:建立高效液相色谱法(HPLC)同时测定风寒感冒颗粒中苦杏仁苷、葛根素和橙皮苷含量的方法。方法:采用Thermo BDS Hypersil-C18色谱柱(250 mm×4.6 mm,5 μm),以甲醇-水为流动相,梯度洗脱;柱温40 ℃。结果:苦杏仁苷、葛根素、橙皮苷线性范围分别为0.01923~0.7691 μg(r=1.0000)、0.02562~1.0250 μg(r=1.0000)、0.006815~0.2726 μg(r=0.9999);平均回收率分别为101.40%(RSD为1.34%,n=9),101.64%(RSD为1.42%,n=9),100.34%(RSD为1.00%,n=9)。结论:该方法简便准确,专属性强,重复性好,可用于风寒感冒颗粒的质量控制。
英文摘要:
      Objective To establish an HPLC method for simultaneous determination of amygdalin,puerarin and hesperidin in Fenghan Ganmao Granules.Methods The test was performed on a Thermo BDS Hypersil-C18 column(250 mm×4.6 mm,5 μm),using methanol-water as mobile phase.The column temperature was 40℃.Results The good linearity was shown in the ranges of 0.01923-0.7691 μg(r=1.0000)、0.02562-1.0250 μg(r=1.0000) and 0.006815-0.2726 μg(r=0.9999) for amygdalin,puerarin and hesperidin respectively.The average recoveries of amygdalin,puerarin and hesperidin were 101.40% with RSD of 1.34%(n=9),101.64% with RSD of 1.42%(n=9) and 100.36% with RSD of 1.00%(n=9) respectively. Conclusion This simple,accurate,specific and reproducible method can be used for the quality control of Fenghan Ganmao Granules.
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