| 蒋健.HPLC法同时测定风寒感冒颗粒中苦杏仁苷、葛根素和橙皮苷的含量[J].中国药品标准,2019,20(6):482-485 |
| HPLC法同时测定风寒感冒颗粒中苦杏仁苷、葛根素和橙皮苷的含量 |
| Simultaneous Determination of Amygdalin，Puerarin and Hesperidin in Fenghan Ganmao Granules by HPLC |
| 投稿时间：2019-02-14 修订日期：2019-11-26 |
| DOI： |
| 中文关键词: 风寒感冒颗粒 苦杏仁苷 葛根素 橙皮苷 高效液相色谱法 |
| 英文关键词: Fenghan Ganmao Granules amygdalin,puerarin,hesperidin HPLC |
| 基金项目: |
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| 摘要点击次数: 875 |
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| 中文摘要: |
| 目的：建立高效液相色谱法(HPLC)同时测定风寒感冒颗粒中苦杏仁苷、葛根素和橙皮苷含量的方法。方法：采用Thermo BDS Hypersil-C18色谱柱(250 mm×4.6 mm,5 μm),以甲醇-水为流动相,梯度洗脱；柱温40 ℃。结果：苦杏仁苷、葛根素、橙皮苷线性范围分别为0.01923~0.7691 μg(r=1.0000)、0.02562~1.0250 μg(r=1.0000)、0.006815~0.2726 μg(r=0.9999)；平均回收率分别为101.40%(RSD为1.34%,n=9),101.64%(RSD为1.42%,n=9),100.34%(RSD为1.00%,n=9)。结论：该方法简便准确,专属性强,重复性好,可用于风寒感冒颗粒的质量控制。 |
| 英文摘要: |
| Objective To establish an HPLC method for simultaneous determination of amygdalin,puerarin and hesperidin in Fenghan Ganmao Granules.Methods The test was performed on a Thermo BDS Hypersil-C18 column(250 mm×4.6 mm,5 μm),using methanol-water as mobile phase.The column temperature was 40℃.Results The good linearity was shown in the ranges of 0.01923-0.7691 μg(r=1.0000)、0.02562-1.0250 μg(r=1.0000) and 0.006815-0.2726 μg(r=0.9999) for amygdalin,puerarin and hesperidin respectively.The average recoveries of amygdalin,puerarin and hesperidin were 101.40% with RSD of 1.34%(n=9),101.64% with RSD of 1.42%(n=9) and 100.36% with RSD of 1.00%(n=9) respectively. Conclusion This simple,accurate,specific and reproducible method can be used for the quality control of Fenghan Ganmao Granules. |
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