| 周震宇.齐墩果酸片的含量测定[J].中国药品标准,2019,20(6):505-509 |
| 齐墩果酸片的含量测定 |
| Determination of Oleanolic Acid Tablets by HPLC |
| 投稿时间：2019-01-23 修订日期：2019-11-21 |
| DOI： |
| 中文关键词: 齐墩果酸片,含量测定,高效液相色谱法 |
| 英文关键词: oleanolic acid tablets, assay, HPLC method |
| 基金项目: |
|
| 摘要点击次数: 974 |
| 全文下载次数: 155 |
| 中文摘要: |
| 目的：建立齐敦果酸片中齐墩果酸含量测定的方法。方法：采用YMC-Pack ODS-A(250×4.6mm,5μm)色谱柱；以0.1%醋酸铵-乙腈(85:15)为流动相,等度洗脱；流量1.0mL.min-1；检测波长210nm；柱温35℃；进样量10μL。结果：齐墩果酸的浓度在10～1000μg.mL-1范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率为99.97%,其RSD为1.43%(n=9)。结论：该方法简便快速,可用于齐敦果酸片的含量测定。 |
| 英文摘要: |
| Objective: To establish a HPLC method for the determination of oleanolic acid in oleanolic acid tablets. Methods: The separation was performed on a YMC-Pack ODS-A (250×4.6mm,5μm) column at 35℃with isocratice elution program of 0.1% ammonium acetate-acetonitrile (85:15) and the flow rate as 1.0mL.min-1. The detection wavelength was set as 210nm, and the injection volume was 10μL. Results: There was the good linearity in the range of 10～1000μg.mL(r=0.9999) between the peak area and concentration of oleanolic acid. The average recovery was 99.97% with RSD as 1.43% (n=9). Conclusion: The simple, efficient and accurate method can be adopted for the assay of the oleanolic acid tablets. |
|
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|