文章摘要
周震宇.齐墩果酸片的含量测定[J].中国药品标准,2019,20(6):505-509
齐墩果酸片的含量测定
Determination of Oleanolic Acid Tablets by HPLC
投稿时间:2019-01-23  修订日期:2019-11-21
DOI:
中文关键词: 齐墩果酸片,含量测定,高效液相色谱法
英文关键词: oleanolic  acid tablets, assay, HPLC  method
基金项目:
作者单位E-mail
周震宇 苏州市食品药品检验所 zhou_198209@163.com 
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中文摘要:
      目的:建立齐敦果酸片中齐墩果酸含量测定的方法。方法:采用YMC-Pack ODS-A(250×4.6mm,5μm)色谱柱;以0.1%醋酸铵-乙腈(85:15)为流动相,等度洗脱;流量1.0mL.min-1;检测波长210nm;柱温35℃;进样量10μL。结果:齐墩果酸的浓度在10~1000μg.mL-1范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率为99.97%,其RSD为1.43%(n=9)。结论:该方法简便快速,可用于齐敦果酸片的含量测定。
英文摘要:
      Objective: To establish a HPLC method for the determination of oleanolic acid in oleanolic acid tablets. Methods: The separation was performed on a YMC-Pack ODS-A (250×4.6mm,5μm) column at 35℃with isocratice elution program of 0.1% ammonium acetate-acetonitrile (85:15) and the flow rate as 1.0mL.min-1. The detection wavelength was set as 210nm, and the injection volume was 10μL. Results: There was the good linearity in the range of 10~1000μg.mL(r=0.9999) between the peak area and concentration of oleanolic acid. The average recovery was 99.97% with RSD as 1.43% (n=9). Conclusion: The simple, efficient and accurate method can be adopted for the assay of the oleanolic acid tablets.
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