| 吴珺,钱忠义.HPLC法测定消旋山莨菪碱滴眼液中苯扎溴铵的含量[J].中国药品标准,2019,20(2):160-163 |
| HPLC法测定消旋山莨菪碱滴眼液中苯扎溴铵的含量 |
| Determination of Benzalkonium Bromide in Raceanisodamine |
| 投稿时间：2019-01-21 修订日期：2019-04-08 |
| DOI： |
| 中文关键词: 消旋山莨菪碱滴眼液 苯扎溴铵 高效液相色谱法 含量测定 |
| 英文关键词: Raceanisodamine eye drops Benzalkonium bromide HPLC Assay |
| 基金项目: |
|
| 摘要点击次数: 653 |
| 全文下载次数: 211 |
| 中文摘要: |
| 目的: 建立高效液相色谱法测定消旋山莨菪碱滴眼液中苯扎溴铵的含量。方法:色谱柱：Waters SunFireTM C18柱（250mm×4.6mm,5μm）；流动相：乙腈-三乙胺磷酸溶液（取三乙胺4mL和磷酸7mL，加水稀释至1000mL）（60：40）；流速1mL?min-1；检测波长215 nm。结果:苯扎溴铵的浓度在0.01362 ～0.1090 mg?moL-1范围内与色谱峰面积呈现良好的线性关系，（r=0. 9999），平均回收率为99.4%，RSD%=0.3%。结论: 本法测定消旋山莨菪碱滴眼液中抑菌剂苯扎溴铵，具有简便、准确、重现性好的优点。 |
| 英文摘要: |
| Objective: To establish a method for the determination of benzalkonium bromide in Raceanisodamine Eye Drops by HPLC. Methods:The chromatographic column is: Waters SunFireTM C18 column (250mm*4.6mm, 5μm). Mobile phase: ace-triethylamine phosphoric acid solution (take 4mL triethylamine and 7 mL phosphoric acid, add water and dilute to 1000mL) (60:40); Flow rate: 1mL?min-1; Detection wavelength: 215 nm. Results:The concentration of benzalkonium bromide in the range of 0.01362 ~ 0.1090 mg?moL-1 has a good linear relationship with the chromatographic peak area (r=0.09999), with an average recovery rate of 99.4% and RSD%= 0.3%. Conclusion: This method is simple ,accurate and highly repeatable for the determination of benzalkonium bromide in raceanisodamine eye drops . |
|
查看/发表评论 下载PDF阅读器 |
| 关闭 |