| 任菲菲,张静,刘德丽,闫雪,郑艳青.HPLC测定小儿感冒颗粒中绿原酸、木犀草苷3,5-O-二咖啡酰基奎宁酸、连翘苷的含量[J].中国药品标准,2019,20(4):325-331 |
| HPLC测定小儿感冒颗粒中绿原酸、木犀草苷3,5-O-二咖啡酰基奎宁酸、连翘苷的含量 |
| Determination of Chlorogenic Acid、Luteolin、3,5-O-Dicaffeoyl Quinic Acid andForsythin in Xiaoerganmao Granules by HPLC |
| 投稿时间：2019-01-18 修订日期：2019-06-26 |
| DOI： |
| 中文关键词: 小儿感冒颗粒 高效液相色谱法 绿原酸 木犀草苷 3,5-O-二咖啡酰基奎宁酸 连翘苷 |
| 英文关键词: Xiaoerganmao Granules Dual wavelength Chlorogenic Acid Luteolin 3,5-O-Dicaffeo- |
| 基金项目: |
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| 摘要点击次数: 731 |
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| 中文摘要: |
| 目的 建立双波长HPLC法测定小儿感冒颗粒中绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸和连翘苷的含量。方法：Agilent C18 色谱柱(4.6mm╳250mm,5μm)；流动相：乙腈-0.4%磷酸,梯度洗脱；检测波长：0-45min(327nm),45-83min(202nm)；柱温：30℃；流速：1mL/min,进样量10μL。结果：绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸、连翘苷浓度分别在 0.01267～0.3801μg/m L、0.1040～1.5600μg/mL、0.00840～0.2526μg/mL、37.90～319.021μg/mL范围内呈良好的线性关系(r=0.9999,1.0000,0.9966,1.0000)；平均加样回收率分别为97.89％、96.37％ 、96.63％和103.3％(n=6)。结论：所建立的双波长HPLC测定法简便、准确、重复性好、专属性强,可同时测定小儿感冒颗粒中的多种有效成分的含量。 |
| 英文摘要: |
| Abstract Objective: To establish a method for determination Chlorogenic Acid、Luteolin、3,5-O-Dicaffeoyl Quinic Acid and Forsythin in Xiaoerganmao Granules by dual-wavelength HPLC.Methods： Agilent C18 (4.6 mm x 200 mm, 5 μm) .The mobile phase was acetonitrile-0.4% phosphate with gradient elutionthe. The column temperature was 30℃.The detection wavelength was set as 327 nm (Chlorogenic Acid、Luteolin、 3,5-O-Dicaffeoyl Quinic Acid)，202 nm (forsythin) and the flow rate was 1.0 mL ? min-1 .Results：The linear ranges of Chlorogenic Acid、Luteolin、3,5-O-Dicaffeoyl Quinic Acid and Forsyhin were between 0.01267-0.3801μg?m L-1 (r=0.9999), 0.1040-1.5600μg?m L-1 mL(r=1.0000),0.0084
0-0.2526μg?mL-1 (r=0.9966),0.03190-0.3190μg?m L-1 mL(r=1.0000)．The average recoveries (n
= 6) were 97.89％,96.37％, 96.63％,103.3％，respectively.Conclusion：The method is simple,accurate and reproducible,and it can be used for quality control of Xiaoerganmao Granules . |
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