| 周绪云.一测多评法同时测定水蛭中4种有效成分的含量[J].中国药品标准,2019,20(4):339-345 |
| 一测多评法同时测定水蛭中4种有效成分的含量 |
| Simultaneous Determination of Four Active Components in Whitmania Pigra by QAMS Method |
| 投稿时间：2018-12-28 修订日期：2019-06-27 |
| DOI： |
| 中文关键词: 一测多评法 水蛭 相对校正因子 外标法 含量测定 |
| 英文关键词: QAMS Whitmania Pigra Relative correction factor External standard method content determination |
| 基金项目: |
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| 中文摘要: |
| 目的：建立同时测定水蛭药材中4 种主要成分含量的方法。方法：采用高效液相色谱法,以次黄嘌呤为内标,计算其对尿嘧啶、黄嘌呤和尿苷的相对校正因子(RCF),通过RCF计算上述3种成分的含量；以外标法测定的含量作实测值,比较计算值与实测值的差异。结果：尿嘧啶、次黄嘌呤、黄嘌呤和尿苷检测质量浓度线性范围分别为1.60～32.00 mg/ml、4.05～81.00 mg/ml、1.50～30.00 mg/ml、1.25～25.00 mg/ml(r>0.999)；精密度、稳定性、重复性试验的RSD＜2.0％；加样回收率试验的RSD 分别为95.05％～99.45％(RSD＝1.73％,n＝6)、95.89％～99.38％(RSD＝1.52％,n＝6)、94.00％～97.88％(RSD＝1.74％,n＝6)、96.07％～99.35％(RSD＝1.18％,n＝6)。计算值与实测值差异无统计学意义(P＞0.05)。结论：该方法操作简便,精密度、稳定性、重复性良好,可用于水蛭药材中4种有效成分含量的同时测定。 |
| 英文摘要: |
| ABSTRACT OBJECTIVE: To establish the method for simultaneous determination of four active components in Whitmania pigra. METHODS: Using hypoxanthine as a reference，HPLC method was adopted to calculate the relative correction factor（RCF）of it to nracil, xanthine and uridine. The contents of above 3 components were calculated through RCF. Using the contents of components determined by external standard method as measured value, the calculated value was compared with measured value. RESULTS: The linear ranges of nracil, hypoxanthine, xanthine and uridine were 1.60-32.00 ?g/ml, 4.05-81.00 ?g/ml, 1.50-30.00 ?g/ml, 1.25-25.00 ?g/ml (r?0.999). respectively. RSDs of precision, stability and reproducibility tests were all lower than 2.0％. The recoveries were 95.05％-99.45％（RSD＝1.73％, n＝6）, 95.89％-99.38％（RSD＝1.52％, n＝6）, 94.00％-97.88％（RSD＝1.74％, n＝6）, 96.07％-99.35％（RSD＝1.18％, n＝6）, respectively. There was no statistical significance between calculated value and measured value（P＞0.05）. CONCLUSIONS: The method is simple, precise, stable and reproducible. It can be used for the determination of Active Components in Whitmania pigra. |
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