| 张筱,齐衍超,杨丽蓉,聂晓华.复方甘草片中甘草酸含量测定方法的改进[J].中国药品标准,2019,20(2):133-137 |
| 复方甘草片中甘草酸含量测定方法的改进 |
| Improvement for content determination of glycyrrhizic acid in compound liquorice Tablets |
| 投稿时间：2018-11-13 修订日期：2019-04-02 |
| DOI： |
| 中文关键词: HPLC 复方甘草片 甘草酸 含量测定 |
| 英文关键词: HPLC Compound liquorice tablets Glycyrrhizic acid Content determination |
| 基金项目: |
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| 摘要点击次数: 636 |
| 全文下载次数: 233 |
| 中文摘要: |
| 目的：改进药典色谱条件,建立HPLC法测定复方甘草片中甘草酸含量测定的方法。方法：Shim-pack GIST C18色谱柱(4.6 mm ×250mm,5μm),流动相：0.025 mol.L-1磷酸二氢钾溶液-0.0025mol.L-1庚烷磺酸钠水溶液-乙腈(36:37:27),检测波长：250nm,柱温：35℃,流速1.0mL.min-1,进样量：10μL,以峰面积外标法定量。结果：甘草酸浓度在15.01～150.1μg. mL-1范围内呈较好线性关系,回归方程为y=8.823x+35.60(R2=0.999),平均回收率为100.6%,RSD=1.26%(n=6)。结论：此方法适用于测定复方甘草片中甘草酸的含量。 |
| 英文摘要: |
| Objective：To improve the chromatographic conditions of Pharmacopoeia and establish a method for the content determination of glycyrrhizic acid in Compound Liquorice Tablets by HPLC. Methods：Shim-pack GIST C18 (4.6 mm × 150 mm) separation column was used with the column temperature at 35℃. The mobile phase was 0.025 mol?L-1 KH2PO4-0.0025 mol? L-1 C7H15NaO3S-acetonitrile (36:37:27). The flow rate was 1.0 mL?min-1 and the UV detection wavelength was at 250nm. The sample volume was 10μL. The external standard method of peak area was employed for quantitative analysis. Results：A good linearity was obtained in the range of 15.01～150.1μg ? mL-1, regression: y=8.823x+35.60(R2=0.999), the average recovery was 100.6%, RSD = 1.26%( n = 6). Conclusion: This method is good for the content determination of glycyrrhizic acid in compound liquorice tablets. |
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