| 章为,李文波,王皖,李帅,李晓燕,刘雁鸣,曾令贵.HPLC法测定浓维磷糖浆3种成分的含量[J].中国药品标准,2018,19(1):58-63 |
| HPLC法测定浓维磷糖浆3种成分的含量 |
| Determination of 3 components in Concentrated Divitamins and Sodium Phosphate Syrup by HPLC |
| 投稿时间：2017-11-15 修订日期：2018-02-08 |
| DOI： |
| 中文关键词: 高效液相色谱法 浓维磷糖浆 咖啡因 维生素B1 烟酸 含量测定 |
| 英文关键词: HPLC Concentrated Divitamins and Sodium Phosphate Syrup Caffeine Vitamin B1 Niacin Assay |
| 基金项目: |
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| 摘要点击次数: 666 |
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| 中文摘要: |
| 目的：建立同时测定浓维磷糖浆中咖啡因、维生素B1和烟酸含量HPLC方法。方法：采用UltiMate XB-C18柱(250mm×4.6mm,5μm)色谱柱；以0.1%辛烷磺酸钠(含0.1%磷酸)为流动相A、乙腈为流动相B,梯度洗脱；流量1.0 mL.min-1；柱温30℃；检测波长261nm。结果：咖啡因、维生素B1和烟酸的线性范围分别为38.22μg/ml～382.25μg.mL-1(r=1.0000)、7.28μg/ml～72.75μg.mL-1(r=0.9995)和0.78μg/ml～7.84μg.mL-1(r=1.0000)；平均回收率(n=9)分别为99.3%、99.8%、98.6%,RSD分别为0.66%、1.14%、1.30%。结论：所建方法快速、准确、可靠,可用于浓维磷糖浆的质量控制。 |
| 英文摘要: |
| Objective: To develop an HPLC method for the determination of Caffeine, Vitamin B1 and Niacin in Concentrated Divitamins and Sodium Phosphate Syrup. Methods: An UltiMate XB-C18 (250mm×4.6mm,5μm) column was adopted. The gradient elution with 0.1% sodium octane sulfonate (with 0.1% phosphoric acid) as mobile phase A and acetonitrile as mobile phase B．The flow rate was set as 1.0 mL.min-1, column temperature as 30℃ and detection wavelength as 261 nm. Results: The linearity ranges of Caffeine, Vitamin B1 and Niacin were 38.22μg/ml-382.25μg.mL-1(r=1.0000),7.28μg/ml-72.75μg.mL-1(r=0.9995) and 0.78μg/ml-7.84μg.mL-1(r=1.0000) respectively. The average recovery rates(n=9) were 99.3%, 99.8% and 98.6% with RSD as 0.66%, 1.14% and 1.30%, respectively. Conclusion: The established method is rapid, accurate and reliable which can be used for the quality control of Concentrated Divitamins and Sodium Phosphate Syrup. |
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