| 伏光华.HPLC法测定拉坦前列素滴眼液中15S-拉坦前列素的含量[J].中国药品标准,2017,18(4):288-293 |
| HPLC法测定拉坦前列素滴眼液中15S-拉坦前列素的含量 |
| HPLC Determination of 15S-Latanoprost in Latanoprost Eye Drops |
| 投稿时间：2017-06-26 修订日期：2017-08-01 |
| DOI： |
| 中文关键词: 拉坦前列素滴眼液 15S-拉坦前列素 高效液相色谱法 含量测定 |
| 英文关键词: Latanoprost Eye Drops Latanoprost HPLC content determination |
| 基金项目: |
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| 摘要点击次数: 545 |
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| 中文摘要: |
| 目的：建立拉坦前列素滴眼液中杂质15S-拉坦前列素的含量测定方法。方法：色谱柱为ZORBAX SB- C18(4.6 mm′250 mm,5 mm),流动相为甲醇-乙腈-水(用冰乙酸调节pH3.0)(56∶14∶30),流速为1.0 ml.min-1,检测波长为210 nm,进样量为100 ml。结果：在选定的色谱条件下,15S-拉坦前列素杂质与主成分可以有效分离,在0.04994～0.9988 mg.mL-1质量浓度范围内呈良好的线性关系(r=0.9993),平均回收度为99.03%(n=9)。结论：所建立的方法可以用于拉坦前列素滴眼液中15S-拉坦前列素的含量测定。 |
| 英文摘要: |
| Objective: To establish an HPLC method for the determination of 15S-Latanoprost in Latanoprost Eye Drops. Method: HPLC separation was carried on a ZORBAX SB- C18 column (4.6 mm′250 mm,5 mm) with the mobile phase consisted of methanol–acetonitrile-water (pH was adjusted to 3.0 with acetic acid)( 56∶14∶30), the flow rate was 1.0ml.min-1 and the detection wavelength was set at 210 nm, and the injection volume was 100 ml. Results: 15S- Latanoprost and Latanoprost were separated efficiently under the selected HPLC conditions, and the method has a good linearity in the range of 0.04994-0.9988 mg.mL-1(r=0.9993)for 15S-Latanoprost. The average recovery of 15S- Latanoprost was 99.03% (n=9). Conclusion: The method can be used for the detection of Latanoprost in Latanoprost Eye Drops. |
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