| 潘雯,陈伟盛.对耐胆盐革兰氏阴性菌检验采用MPN法和平板法的比较[J].中国药品标准,2017,18(3):199-201 |
| 对耐胆盐革兰氏阴性菌检验采用MPN法和平板法的比较 |
| A comparison of the enumeration of bile-tolerant gram-negative bacteria with MPN method and plate method |
| 投稿时间：2017-02-28 修订日期：2017-06-05 |
| DOI： |
| 中文关键词: 耐胆盐革兰氏阴性菌 MPN法 平板法 |
| 英文关键词: Bile-tolerant gram-negative bacteria MPN method plate method |
| 基金项目: |
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| 摘要点击次数: 1546 |
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| 中文摘要: |
| 目的：比较MPN法和平板法对耐胆盐革兰氏阴性菌计数是否存在显著性差异。方法：采用单人四种方法计数和多人两种方法计数,通过统计学分析是否存在显著性差异。结果： MPN的离散性较大,预培养5小时较2小时菌体有增殖。结论：检查耐胆盐革兰氏阴性菌应尽量在2小时内完成,MPN法较灵敏,更适合于污染轻的样品。 |
| 英文摘要: |
| Object: To compare the MPN method and plate method on enumeration of bile-tolerant gram-negative bacteria. Method: The statistical analyses of the enumerations between MPN method and plate method were used on one-researcher and multi-researchers. Result: The measure of dispersion in the enumeration data from MPN method was greater than plate method. And compared with 2h pre-incubation, bacteria proliferation was found more after 5h pre-incubation in MPN method. Conclusion: The MPN method was more sensitive than plate method so that it was applicable to enumerate bile-tolerant gram-negative bacteria from the sample with a little microbial contamination, and the bacteria should be resuscitated for 2h pre-incubation at most. |
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