| 黄海燕,饶伟文,钟建理,韦宜欢.银杏叶提取物及其制剂中非法添加染料木素的检测[J].中国药品标准,2016,17(5):353-357 |
| 银杏叶提取物及其制剂中非法添加染料木素的检测 |
| Determination of Genistein in Ginkgo Leaves Extract and Its Preparation |
| 投稿时间：2016-04-22 修订日期：2016-08-23 |
| DOI： |
| 中文关键词: 银杏叶 银杏叶提取物 银杏叶制剂 染料木素 高效液相色谱-二极管阵列检测器 |
| 英文关键词: Ginkgo leaf Ginkgo leaves extract Ginkgo leaves preparation genistein HPLC-DAD |
| 基金项目: |
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| 中文摘要: |
| 目的：对银杏叶提取物及其制剂中非法添加染料木素进行鉴定和检测。方法：采用HPLC-DAD法，色谱柱为DIKMA Inertsil ODS-3 (4.6mm×250mm，5μm)，流动相为甲醇-0.4%磷酸溶液(50:50)，流量1.0ml?min-1，柱温35℃，检测波长260nm，并同时采集210～400nm的光谱图，对银杏叶、银杏叶提取物及其制剂中的染料木素进行定性分析。结果：部分银杏叶提取物和银杏叶制剂样品HPLC色谱图中出现与染料木素对照品保留时间一致的色谱峰，DAD光谱一致。结论：部分厂家生产的银杏叶提取物及其制剂中含有染料木素，为生产时添加了其他含染料木素的原料。 |
| 英文摘要: |
| Objective: To identify and determine Genistein illegally added in Ginkgo Leaves Extract and its preparation. Methods: HPLC-DAD was used on a column of DIKMA Inertsil ODS-3 (4.6mm ×250 mm, 5μm) with the mobile phase of methanol-0.4% phosphoric acid solution (50:50) at the flow rate of 1.0 ml?min-1 , the column temperature was set at 35℃and the detection wavelength was 260nm.The spectrogram within 200～400nm wavelength was collected simultaneously . Results: Chromatographic peaks coincided with Genistein were found in several batches of Ginkgo Leaves Extract and its preparation from different manufactures . Conclusion: Several batches of Ginkgo Leaves Extract and its preparation t contain Genistein which may be artificially added. |
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