| 夏瑞,代红,车宝泉,张喆,郭洪祝.HPLC法测定盐酸洛贝林注射液中的有关物质[J].中国药品标准,2009,10(1):46-50 |
| HPLC法测定盐酸洛贝林注射液中的有关物质 |
| Determination of Related Substances in Lobeline Hydrochloride Injection by RP-HPLC |
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| DOI： |
| 中文关键词: 盐酸洛贝林 高效液相色谱法 有关物质 |
| 英文关键词: Lobeline Hydrochloride HPLC Related Substances |
| 基金项目: |
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| 摘要点击次数: 573 |
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| 中文摘要: |
| 目的: 采用反相高效液相色谱法测定盐酸洛贝林注射液中的有关物质。方法: 采用反相高效液相色谱法,C18柱,流动相为0.05mol·L-1磷酸二氢钾溶液(用磷酸调节pH值至3.0)-甲醇(55:45)为流动相;检测波长为210nm。结果: 盐酸洛贝林注射液中除主峰(顺式洛贝林)外,出现一较大含量的色谱峰,为洛贝林的异构体(反式洛贝林);盐酸洛贝林与其他杂质分离良好,样品溶液在8h内稳定;结论: 所建方法能有效地分离盐酸洛贝林注射液中的有关物质,方法简单、可靠。 |
| 英文摘要: |
| Objective: To develop a method for determination of related substance in Lobeline Hydrochloride Injection by RP-HPLC.Method: The analysis was performed on a C18 column,using mobile phase consisted of 0.05mol/L potassium dihydrogen phosphate solution(adjusted to pH 3.0 with phosphoric acid)-methanol(55:45).The detection wavelength was 210 nm.Results: There were a big content chromatograph peak of trans-(-)-Lobeline and the prominent peak which is cis-(-)-Lobeline in Lobeline Hydrochloride Injection.Lobeline Hydrochloride and related substances could be separated well.Samples were stable in 8 hrs.Conclusion: This method can be used to simultaneously separate and determine the related substances in Lobeline Hydrochloride Injection.It is simple and accurate. |
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