| 赵啸虎,郭美玲,张红梅.HPLC法测定小儿白贝止咳糖浆中原阿片碱的含量[J].中国药品标准,2019,20(2):156-159 |
| HPLC法测定小儿白贝止咳糖浆中原阿片碱的含量 |
| Determination of Protopine in the Xiao’er Baibei Zhike syrup by HPLC |
| 投稿时间：2018-12-03 修订日期：2019-04-02 |
| DOI： |
| 中文关键词: HPLC 小儿白贝止咳糖浆 原阿片碱 |
| 英文关键词: HPLC Xiao’er Baibei Zhike syrup protopine |
| 基金项目: |
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| 摘要点击次数: 838 |
| 全文下载次数: 176 |
| 中文摘要: |
| 目的：建立小儿白贝止咳糖浆中原阿片碱的含量测定方法。方法：采用高效液相色谱法测定，色谱条件为：Agilent TC C18 色谱柱(250mm×4.6 mm，5 μm)，流动相为乙腈-三乙胺醋酸溶液（18 : 82），流速1 mL? min-1，检测波长289 nm，柱温30 ℃。结果：原阿片碱在0.1395～2.2323 μg范围内线性关系良好（r = 0.999 8），平均回收率99.14 %（RSD=0.82 %）。3批样品含量测定结果：平均含量为6.6 μg?mL-1。结论：本方法简便、准确、重现性好，可以作为小儿白贝止咳糖浆的控制方法。 |
| 英文摘要: |
| Objective: To establish a method for content determination of protopine in Xiao’er Baibei Zhike syrup. Methods: The HPLC system consisting of a Agilent TC C18 column(250mm×4.6 mm,5 μm) and a mixture of acetonitrile-acetic triethylamine solution (18:82) as the mobile phase was adopted.The flow rate was 1.0 mL? min-1.The detector wavelength was set at 289 nm and the column temperature was maintained at 30℃. Results: The standard curve of protopine was linear over the range of 0.1395-2.2323μg(r=0.999 8), the average recovery was 99.14 % (RSD=0.82 %). The average content of protopine in 3 batches of samples were 6.6μg?mL-1. Conclusion:The method is simple,accurate,and reproducible,which can be used for the quality control of Xiao’er Baibei Zhike syrup. |
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