文章摘要
朱晓燕,陈伟盛,黄韵璇,侯惠婵.国产与泰国薏苡仁DNA条形码的比较[J].中国药品标准,2018,19(5):325-329
国产与泰国薏苡仁DNA条形码的比较
Comparison of two Coix Semen based on DNA barcoding technique
投稿时间:2018-03-22  修订日期:2018-09-29
DOI:
中文关键词: 国产薏苡仁  泰国薏苡仁  DNA条形码  亲缘关系  序列比对
英文关键词: Chinese Coix Semen  imported Coix Semen  DNA barcoding  genetic relationship  sequence comparison
基金项目:
作者单位E-mail
朱晓燕* 广州市药品检验所 xiaoyan_2720@163.com 
陈伟盛 广州市药品检验所  
黄韵璇 广州市药品检验所  
侯惠婵 广州市药品检验所  
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中文摘要:
      目的:通过对国产薏苡仁和泰国薏苡仁五段DNA条形码序列的比较,探索两者的亲缘关系,为其基因型上的差别提供佐证。方法:采用聚合酶链式反应(PCR)扩增两种薏苡仁的ITS2、psbA-trnH、rbcL、matK和ITS五段保守基因,并对其PCR产物进行测序和比对。结果:五段基因的PCR扩增和测序成功率均为100%,且国产薏苡仁与泰国薏苡仁的五段基因序列无位点差异。结论:国产薏苡仁与泰国薏苡仁的亲缘关系极近,或可一定程度上替代国产薏苡仁。
英文摘要:
      Objective: Through the comparison of five DNA barcoding of Chinese Coix Semen and imported Coix Semen, to investigate the genotypic difference between them. Method: Amplify ITS2, psbA-trnH, rbcL, matK and ITS of two Coix Semen, then analyze their DNA sequence. Result: The PCR and sequencing success rate of the five regions was 100%, and there is no site difference between Chinese Coix Semen and imported Coix Semen. Conclusion: The genetic relationship of Chinese Coix Semen and imported Coix Semen is presumed to be very close, so the imported Coix Semen may sometimes be substituded for Chinese Coix Semen.
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