| 李浩然,万彦,付小六.反向离子对色谱法测定氨甲苯酸片的含量[J].中国药品标准,2018,19(1):54-57 |
| 反向离子对色谱法测定氨甲苯酸片的含量 |
| Determination of aminomethylbenzoic acid tablets by reversed phase ion pair RP-HPLC |
| 投稿时间：2017-11-06 修订日期：2018-02-07 |
| DOI： |
| 中文关键词: 反向离子对色谱法 氨甲苯酸片 含量测定 |
| 英文关键词: Reverse phase ion pair chromatography Aminomethylbenzoic Acid Tablets Assay |
| 基金项目: |
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| 摘要点击次数: 777 |
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| 中文摘要: |
| 目的:建立反向离子对色谱法测定氨甲苯酸片中氨甲苯酸含量的方法。方法:采用Agilent 5TC-C18 150mm×4.6μm色谱柱，用甲醇-0.005mol?L-1的十二烷基硫酸钠溶液（用磷酸调节pH值至3.0）=55:45为流动相，流速1.0mL?min-1,柱温30℃，检测波长226nm。结果:氨甲苯酸在约1.0～0.02mg?mL-1的浓度范围内线性关系良好（r=0.9999）；平均回收率为99.96%，RSD为0.15%（n=9），样品测定误差<0.1%。结论:本法经方法学验证，可用于氨甲苯酸片的质量控制。 |
| 英文摘要: |
| Abstract Objective:To establish phase ion pair RP-HPLC methods for the determination of the concentration of aminomethylbenzoic acid tablets.Methods:The conventration of aminomethylbenzoic acid tablets were analyzed by phase ion pair RP-HPLC,theseparation was performed in a Agilent 5TC-C18 150mm×4.6μm column,with mobile methanol and 0.005mol?L-1 lauryl sulfatesolution (phosphoric acidregulate pH3.0)=55:45, the current speed was 1.0mL?min-1,column temperature was 30℃,the UV-detector at 226nm was selected.Result:The calibration curve was in good linearity over the range between about 1.0mg?mL-1and 0.02mg?mL-1 (r=0.9999),the average recovery was 99.96%and RSD was 0.15%(n=9）,the measurable sample deviation<0.1%.Conclusion:The method is verificated and can be used for quality control of aminomethylbenzoic acid tablets. |
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