| 郭增光,郭增光,罗晓倩,陈仁燕.心可宁胶囊中华蟾酥毒基和酯蟾毒配基的HPLC测定[J].中国药品标准,2017,18(6):435-438 |
| 心可宁胶囊中华蟾酥毒基和酯蟾毒配基的HPLC测定 |
| Study on the Determination of Cinobufagin and Resibufogenin in Xinkening Capsules by HPLC |
| 投稿时间：2017-07-19 修订日期：2017-12-11 |
| DOI： |
| 中文关键词: 心可宁胶囊 HPLC 华蟾酥毒基 酯蟾毒配基 |
| 英文关键词: xinkening capsules HPLC cinobufagin resibufogenin |
| 基金项目: |
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| 中文摘要: |
| 目的：建立HPLC法测定心可宁所含蟾酥中华蟾酥毒基和酯蟾毒配基的方法,以更好的控制心可宁胶囊的质量。方法：采用Thermo C18色谱柱,以0.5%磷酸二氢钾溶液-乙腈(50:50)为流动相,用磷酸调节pH至3.2,检测波长为296 nm。结果：华蟾酥毒基在30.72～71.68 μg /mL范围内呈良好的线性关系(r=1),酯蟾毒配基在30.78～71.82 μg /mL范围内呈良好的线性关系(r=0.9999),蟾酥平均回收率为99.43%,RSD为0.78%。结论：表明本方法准确、有效,可以作为质控检测方法。 |
| 英文摘要: |
| Objective: A HPLC method for the determination of cinobufagin and resibufogenin in Xinkening capsules was established to control the drug quality. Methods: The determination was carried out using an Thermo C18 column with a solvent system: 0.5% monopotassium phosphate-acetonitrile (50:50). PH was adjusted to 3.2 with phosphoric acid and the detection wavelength was 296 nm. Results: The linear ranges of cinobufagin was 30.72～71.68 μg/mL (r=1) and the linear ranges of resibufogenin was 30.78～71.82 μg/mL (r=0.9999). The average recovery of venenum bufonis was 99.43% and the RSD was 0.78%. Conclusion: It showed that this method was accurate and effective, and could be used as a quality control method. |
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