高威,张颖,陈尚昆.HPLC-ELSD法测定芪冬养血胶囊中黄芪甲苷的含量[J].中国药品标准,2017,18(3):190-193 |
HPLC-ELSD法测定芪冬养血胶囊中黄芪甲苷的含量 |
Determination of Astragaloside Ⅳ in Qidong Yangxue Capsules by HPLC-ELSD |
投稿时间:2017-04-01 修订日期:2017-05-12 |
DOI: |
中文关键词: 黄芪甲苷 高效液相色谱法 芪冬养血胶囊 含量测定 |
英文关键词: astragaloside Ⅳ HPLC Qidong Yangxue Capsules Determination |
基金项目: |
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中文摘要: |
摘要 目的:建立芪冬养血胶囊中黄芪甲苷的HPLC-ELSD含量测定方法。方法:采用高效液相色谱法对芪冬养血胶囊中的黄芪甲苷含量进行测定。色谱柱为Agela-Venusil MP C18 (4.6mm×250mm,5μm),流动相为乙腈-水(36:64),等度洗脱,流速1.0mL·min-1,柱温:40℃,漂移管温度:85℃,空气流速1.7L·min-1。结果:黄芪甲苷在0.997μg-9.97μg进样量对数值与峰面积的对数值呈良好的线性关系(R=0.9996),平均加样回收率为95.22%,RSD1.5%(n=6)。结论:本实验建立的方法简便快捷,适用于该制剂中黄芪甲苷的含量测定。 |
英文摘要: |
Abstract Objective:To establish HPLC-ELSD method for assay of astragaloside Ⅳ in Qidong Yangxue Capsules.Method:The content of astragaloside Ⅳ was determined by a HPLC method.The Agela-Venusil MP C18 (4.6mm×250mm,5μm)was used.The mobile phase was acetonitrile-water(36:64) with 1.0mL·min-1 flow rate at 40℃.The temperature of drift tube for ELSD was set at 85℃,the air flow rate was 1.7L·min-1.Result:The linear range of astragaloside Ⅳ was 0.997μg-9.97μg(R=0.9996).The average recovery was 95.22% (RSD1.5%).Conclusion:The method is simple and quick.It can be used as determination method of astragaloside Ⅳ in Qidong Yangxue Capsules. |
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