| 荆汉卫,石炳娟,陈志恒,辛英芝,郭琪.HPLC-ELSD法同时测定三黄片中的四种目标成分[J].中国药品标准,2017,18(3):207-210 |
| HPLC-ELSD法同时测定三黄片中的四种目标成分 |
| Simultaneous determination of four target components in Sanhuang Tablet by HPLC - ELSD method |
| 投稿时间：2017-03-20 修订日期：2017-06-08 |
| DOI： |
| 中文关键词: 三黄片 大黄素 大黄酚 盐酸小檗碱 黄芩苷 目标成分 HPLC-ELSD 含量测定 |
| 英文关键词: Sanhuang Tablet Emodin Chrysophanol Berberine hydrochloride Baicalin Target composition HPLC-ELSD Conten determination |
| 基金项目: |
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| 摘要点击次数: 358 |
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| 中文摘要: |
| 目的：建立HPLC-ELSD法同时测定三黄片中大黄素、大黄酚、盐酸小檗碱、黄芩苷含量的方法。方法：采用HPLC-ELSD法,以C18柱(250 mm×4.6 mm,5 μm)为色谱柱,以乙腈(A)和0.02mol/mL乙酸铵水溶液(B)为流动相,进行梯度洗脱,流速为1.0ml/min,柱温40℃,漂移管温度80℃,氮气流量为1.2ml/min。结果：四种成分分离效果好,精密度高,重复性良好,回收率符合要求。结论：该方法可以用于同时测定三黄片中的四种目标成分的含量。 |
| 英文摘要: |
| Objective:to establish a method for simultaneous determination of emodin, chrysophanol, berberine hydrochloride and baicalin in Sanhuang Tablet by HPLC-ELSD. Methods: using HPLC – ELSD, using C18 column ( 250 mm × 4.6 mm, 5 μm ), Using acetonitrile (A ) and 0.02mol / ml of semiprepatative solution ( B ) as mobile phase, gradient elution was carried out, The flow rate is 1.0 ml / min, column temperature 40 ℃, evaporation tube temperature 80 ℃, nitrogen flow was 1.2 ml / min. Results: the separation effect of four ingredients is good, high precision,repeatability is good,the recovery rate meets the requirements. Conclusion: the method can accurately determine the content of four target ingredients in Sanhuang Tablet. |
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