| 甘永琦,朱斌.乳酶生标准中乳酸鉴别试验的影响因素探讨[J].中国药品标准,2017,18(1):9-12 |
| 乳酶生标准中乳酸鉴别试验的影响因素探讨 |
| Study on the Influence Factors of Lactic Acid Identification in theLactasin Specification |
| 投稿时间：2016-08-03 修订日期：2016-12-29 |
| DOI： |
| 中文关键词: 乳酶生 乳酸鉴别 乳酸含量 乳糖含量 |
| 英文关键词: Lactasin lactic acid identification lactic acid content lactose content |
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| 摘要点击次数: 1060 |
| 全文下载次数: 140 |
| 中文摘要: |
| 目的：考察乳酶生质量标准中乳酸鉴别试验的影响因素。方法：改进现行标准中主要操作步骤，测定不同来源牛奶培养基中乳酸和乳糖的含量。结果：使用不同来源的牛奶培养基，鉴别反应的颜色差异显著；样品稀释倍数由原来的20倍改为5倍后，鉴别反应呈现的颜色最深；未经灭菌处理的牛奶培养基发酵产生的乳酸含量较高，其乳酸鉴别反应颜色最深。结论：牛奶培养基中糖类成分和含量的差异及样品中乳酸浓度是影响乳酸鉴别试验结果判定的主要因素。 |
| 英文摘要: |
| Objective: To investigate the influence factors of lactic acid identification in the Lactasin specification, the Ch.P 2015 edition. Methods: To improve the test procedure of lactic acid identification in the lactasin specification and to determine the contents of lactic acid, and lactose in the milk culture media with different sources. Results: The colors of identification were different significantly with the different sources milk culture media. The deepest color appeared when the sample was diluted 5 times changed from the previous 20 times. The fermentation of unsterilized milk culture medium produced the highest content of lactic acid, and lactic acid identification reaction showed the deepest color accordingly. Conclusion: The main factors influencing the results of lactic acid identification were the differences of carbohydrate composition and content in the milk culture media, and concentration of lactic acid in the sample. |
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