| 郭美玲.窍耳聋丸UPLC特征图谱的研究[J].中国药品标准,2016,17(6):415-417 |
| 窍耳聋丸UPLC特征图谱的研究 |
| Study on Specific Chromatogram of Tongqiao Erlong Pills by UPLC |
| 投稿时间：2016-06-06 修订日期：2016-11-14 |
| DOI： |
| 中文关键词: 通窍耳聋丸 超高效液相色谱 特征图谱 |
| 英文关键词: Tongqiao Erlong pills UPLC Specific chromatogram |
| 基金项目: |
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| 摘要点击次数: 648 |
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| 中文摘要: |
| 目的：用UPLC建立通窍耳聋丸特征图谱的质量评价方法。方法：Acquity UPLC BEH C18色谱柱（2.1 mm×100mm，1.7μm）；流动相为甲醇-0.1%磷酸溶液，采用梯度洗脱；流量0.3ml?min-1；检测波长250nm；柱温35℃。结果：建立的特征图谱信息丰富，7味中药材中的15个特征峰分离良好，且重复性和稳定性的相对标准偏差均小于1.0%。结论：所建立的方法快速、可靠，专属性强，适用于全面控制通窍耳聋丸的质量。 |
| 英文摘要: |
| Objective: To establish an UPLC method of specific chromatogram for the quality evaluation of Tongqiao Erlong Pills. Methods: The separation was performed on an Acquity UPLC BEH C18 column (2.1mm×100mm, 1.7μm), the mobile phase was a mixture of methanol-0.1% phosphoric acid at a flow rate of 0.3 mL?min-1, gradient elution . The wavelength was set at 250nm and the temperature of the column was 35℃. Results: The information of UPLC specific chromatogram was very abundant, 15 components of 7 Chinese herbal medicines could be separated completely, and the RSDs of repeatability and stability were less than 1.0%. Conclusion: The method is fast, simple and reliable, which can be used for the comprehensive quality control of Tongqiao Erlong Pills. |
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