| 张劲松,桑 晶,孙叶丹,匡 荣,洪利娅,许国峰.直接肽段结合方法(DPRA)在中药注射剂过敏反应预测中的应用[J].中国药品标准,2016,17(4):268-271 |
| 直接肽段结合方法(DPRA)在中药注射剂过敏反应预测中的应用 |
| The Application of Direct Peptide Reactivity Assay for Sensitization Prediction of Chinese Medicine Injection |
| 投稿时间：2016-03-09 修订日期：2016-07-19 |
| DOI： |
| 中文关键词: 直接肽段结合方法 中药注射剂 过敏反应 预测 |
| 英文关键词: Direct Peptide Reactivity Assay (DPRA) Chinese medicine injection allergic reaction prediction |
| 基金项目: |
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| 摘要点击次数: 432 |
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| 中文摘要: |
| 目的:采用直接肽段结合方法（DPRA）考察两种中药注射剂的过敏反应，评估该方法在中药注射剂过敏反应检测中应用的可能性。方法:运用HPLC法，通过测定两种中药注射剂对半胱氨酸多肽和赖氨酸多肽的消耗，来推测其产生过敏反应的可能性。结果:两种中药注射剂对半胱氨酸多肽和赖氨酸多肽均有不同程度的消耗，预示有潜在的致敏性。结论:该方法可以作为中药注射剂过敏反应的预测方法。 |
| 英文摘要: |
| Objective: To evaluate the possibility of applying the method of Direct Peptide Reactivity Assay (DPRA) to predict allergic reaction in Chinese medicine injection,two kinds of Chinese medicine Injection were tested. Methods: Depletion of cysteine- and lysine-containing peptide were measured by high performance liquid chromatography (HPLC), which were used in assessment of sensitization for these two Chinese medicine Injections. Results: The results showed that there were some depletion of both cysteine- and lysine-containing peptide with these two Chinese medicine Injections, which indicated that these two Chinese medicine Injections had some potential of allergic reaction. Conclusion: DPRA can be used as a good method in assessment of sensitization for Chinese medicine Injection. |
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