李筱玲,刘敏,李美芳,李玉兰,王铁杰.HPLC法测定愈酚维林片的含量[J].中国药品标准,2016,17(4):263-267 |
HPLC法测定愈酚维林片的含量 |
Determination of Pentoxyverine Citrate and Guaifenesin Tablets by HPLC |
投稿时间:2016-03-09 修订日期:2016-07-18 |
DOI: |
中文关键词: 愈酚维林片 枸橼酸喷托维林 愈创甘油醚 高效液相色谱法 含量测定 |
英文关键词: Pentoxyverine Citrate and Guaifenesin Tablets HPLC content determination |
基金项目: |
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中文摘要: |
目的:建立愈酚维林片的含量测定方法。方法:采用高效液相色谱法, CAPCELL PAK C18 MGⅡ S-5 (4.6 mm×250mm,5μm)色谱柱,以乙腈-磷酸三乙胺缓冲溶液(33:67)为流动相,流量为1.0mL?min-1,柱温为35℃,检测波长为215nm和275nm,分别测定枸橼酸喷托维林及愈创甘油醚的含量。结果:枸橼酸喷托维林在5.1~50.9μg?mL-1的浓度范围内线性良好,线性方程为A = 11 906C – 4 845.4(r= 0.999 9),平均回收率为99.14%(RSD=1.0%,n=9);愈创甘油醚在30.0~300.0μg?mL-1的浓度范围内线性良好,线性方程为A= 13 809 C – 14208(r=1.000),平均回收率为100.25%(RSD=1.4%,n=9)。结论:本方法操作简便,灵敏度高,专属性强,结果准确可靠,可同时测定愈酚维林片中枸橼酸喷托维林和愈创甘油醚的含量。 |
英文摘要: |
Objective:To establish a method for the quantitative analysis of Pentoxyverine Citrate and Guaifenesin Tablets by HPLC. Methods:The analysis was performed on a CAPCELL PAK C18 MGⅡ S-5 column (4.6 mm×250mm,5μm) with a
mobile phase of acetonitrile- triethylammonium phosphate buffer solution(33:67). The detection wavelength was 215nm for Pentoxyverine Citrate and 275nm for Guaifenesin, respectively. The flow rate was 1.0mL?min-1 and the column temperature was maintained at 35 ℃. Results:The linear range of Pentoxyverine Citrate was 5.1~50.91μg?mL-1, A = 11 906C – 4 845.4 (r= 0.999 9), the average recovery was 99.14% (RSD=1.0%,n=9).The linear range of Guaifenesin was 30.0~300.0μg?mL-1, A= 13 809 C – 14 208 (r=1.000), the average recovery was 100.25% (RSD=1.4%,n=9).Conclusion: The method is convenient,high sensitivity, exclusive and reliable.It can be used for simultaneous determination of Pentoxyverine Citrate and Guaifenesin in Pentoxyverine Citrate and Guaifenesin Tablets . |
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