| 郝秋红.浊度法测定制霉素片的含量[J].中国药品标准,2016,17(5):340-344 |
| 浊度法测定制霉素片的含量 |
| Content Determination of Nysfungin Tablets by Turbidimetric Method |
| 投稿时间：2016-01-21 修订日期：2016-06-19 |
| DOI： |
| 中文关键词: 制霉素片 含量 浊度法 |
| 英文关键词: Nysungin Tablets content turbidimetric method |
| 基金项目: |
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| 中文摘要: |
| 摘要 目的:建立浊度法测定制霉素片含量的方法。方法:以白色念珠菌为试验菌，加菌量0.3%（每100mL培养基中加入0.3mL试验菌），35℃培养3～4h，530nm波长处在线测定其吸光度。结果:在4.0～12.0U?mL-1的范围内， 吸光度（A）与浓度对数（lgC）呈良好线性关系，平均回收率为98.9%（RSD=1.2%），浊度法与现行标准管碟法测得的含量结果基本一致。结论：浊度法可代替管碟法作为制霉素片含量的测定方法。 |
| 英文摘要: |
| Objective: To develop a turbidimetric method for the content determination of Nysfungin Tablets. Method: Candida albicans was the test organismsand adjusted to 0.3% ratio (0.3mL test bacteria were added to each 100mL medium) in spore suspension.Absorbance was determined on-line at 530nm after an incubation period of 3-4h at 35℃. Results: In the range of 4.0 ~ 12.0U?mL-1, the absorbance (A) and the concentration logarithm (lgC) showed a good linear relationship, the average recovery was 98.9% with RSD of 1.2%. The result of turbidimetric method was consistent with the result of current standard cup-plate method. Conclusion: Turbidimetric method can replace cup-plate method to determine the content of Nysfungin Tablets. |
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